Use of Enzymatic Biosensors to Quantify Endogenous ATP or H<sub>2</sub>O<sub>2</sub> in the Kidney

Oleg Palygin; Vladislav Levchenko; Louise C. Evans; Gregory Blass; Allen W. Cowley Jr.; Alexander Staruschenko

doi:10.3791/53059

JoVE Journal
Biology

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Use of Enzymatic Biosensors to Quantify Endogenous ATP or H₂O₂ in the Kidney

利用酶生物传感器的量化内源性ATP或H 2 0 2在肾脏

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10:00 min

October 12, 2015

DOI: 10.3791/53059-v

Oleg Palygin¹, Vladislav Levchenko¹, Louise C. Evans¹, Gregory Blass¹, Allen W. Cowley Jr.¹, Alexander Staruschenko¹

¹Department of Physiology,Medical College of Wisconsin

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这些程序的总体目标是测量整个肾脏中 TP 和过氧化氢的实时内源性间质浓度。这是通过首先将离体和体内应用的传感器再水化和校准到已知浓度的 TP 和过氧化氢来实现的。对于离体传感器安装，通过手术对肾脏进行器械作并从血液中清除。

该程序是通过主动脉冲洗肾脏来进行的。然后手术分离大鼠肾脏，置于记录室中并灌注生理盐水。答：使用离体传感器测量 TP 和过氧化氢以进行体内手术。

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分子生物学第104 生物传感器肾 ATP H 2 0 2 大鼠安培嘌呤信号