Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

Yoshikazu Kishino; Tomohisa Seki; Shinsuke Yuasa; Jun Fujita; Keiichi Fukuda

doi:10.3791/53225

Journal

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发育生物学

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从人外周T细胞无饲养条件下，使用仙台病毒诱导多能干细胞的生成

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

JoVE 杂志
发育生物学

This content is Free Access.

JoVE 杂志发育生物学

Generation of Induced Pluripotent Stem Cells from Human Peripheral T Cells Using Sendai Virus in Feeder-free Conditions

从人外周T细胞无饲养条件下，使用仙台病毒诱导多能干细胞的生成

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11,905 Views

•

09:32 min

•

November 11, 2015

DOI:

10.3791/53225-v

DOI:

10.3791/53225-v

•

09:32 min

November 11, 2015

•

4 Views

Yoshikazu Kishino¹, Tomohisa Seki², Shinsuke Yuasa¹, Jun Fujita¹, Keiichi Fukuda¹

¹Department of Cardiology,Keio University School of Medicine, ²Department of Emergency and Critical Care Medicine,Keio University School of Medicine

Summary

This protocol describes how to generate induced pluripotent stem cells (iPSCs) from human peripheral T cells in feeder-free conditions using a combination of matrigel and Sendai virus vectors containing reprogramming factors.

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Induced Pluripotent Stem Cells IPSCs Peripheral T Cells Sendai Virus Feeder-free Conditions Regenerative Therapies T Cell Receptor Antigen-specific T Cells Genome Rearrangement Clinical Application Fetal Bovine Serum Feeder Cells Biosafety Activated T Cells

从人外周T细胞无饲养条件下，使用仙台病毒诱导多能干细胞的生成

Summary

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