Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

Xiaoyu Liu; Yali Sun; Camilla J K&#248;rner; Xinran Du; Marie E. Vollmer; Karolina M. Pajerowska-Mukhtar

doi:10.3791/53364

Journal

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免疫与感染

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细菌性叶渗透试验使用的植物防御反应精细表征拟南芥，丁香假单胞菌病害系统

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the <em>Arabidopsis thaliana-Pseudomonas syringae</em> Pathosystem

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JoVE 杂志免疫与感染

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem

细菌性叶渗透试验使用的植物防御反应精细表征拟南芥，丁香假单胞菌病害系统

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21,777 Views

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11:50 min

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October 01, 2015

DOI:

10.3791/53364-v

DOI:

10.3791/53364-v

•

11:50 min

October 01, 2015

•

21764 Views

Xiaoyu Liu¹, Yali Sun¹, Camilla J Kørner¹, Xinran Du¹, Marie E. Vollmer¹, Karolina M. Pajerowska-Mukhtar¹

¹Department of Biology,University of Alabama at Birmingham

Summary

Quantification of pathogen growth is a powerful tool to characterize various Arabidopsis thaliana (hereafter: Arabidopsis) immune responses. The method described here presents an optimized syringe infiltration assay to quantify the Pseudomonas syringae pv. maculicola ES4326 growth in adult Arabidopsis leaves.