Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

Pamela N. Peters; Elizabeth M. Schryver; Ernst Lengyel; Hilary Kenny

doi:10.3791/53541

Journal

/

医学

/

造型卵巢癌传播在人类腹腔的器官文化的早期步骤

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

JoVE 杂志
医学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志医学

Modeling the Early Steps of Ovarian Cancer Dissemination in an Organotypic Culture of the Human Peritoneal Cavity

造型卵巢癌传播在人类腹腔的器官文化的早期步骤

Please note that all translations are automatically generated. Click here for the English version.

26,535 Views

•

09:00 min

•

December 31, 2015

DOI:

10.3791/53541-v

DOI:

10.3791/53541-v

•

09:00 min

December 31, 2015

•

4 Views

Pamela N. Peters¹, Elizabeth M. Schryver¹, Ernst Lengyel¹, Hilary Kenny¹

¹Department of Obstetrics and Gynecology, Section of Gynecologic Oncology,University of Chicago

Summary

Here, we present a protocol to construct a three-dimensional in vitro model of the lining of the peritoneal cavity, composed of primary human mesothelial cells and fibroblasts layered with extracellular matrix, as a tool to investigate ovarian cancer cell adhesion, invasion, and proliferation.