Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

Dulce Avila-Rodr&#237;guez; Karina Paisano-Cer&#243;n; Irene Valdovinos- Ram&#237;rez; Carmen Solano-Agama; Alma Ortiz-Plata; Mar&#237;a E. Mendoza-Garrido

doi:10.3791/53637

Journal

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医学

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人垂体腺瘤细胞的三维海藻酸钠珠文化

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

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医学

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JoVE 杂志医学

Three-dimensional Alginate-bead Culture of Human Pituitary Adenoma Cells

人垂体腺瘤细胞的三维海藻酸钠珠文化

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9,896 Views

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08:31 min

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February 18, 2016

DOI:

10.3791/53637-v

DOI:

10.3791/53637-v

•

08:31 min

February 18, 2016

•

7 Views

Dulce Avila-Rodríguez¹, Karina Paisano-Cerón¹, Irene Valdovinos- Ramírez¹, Carmen Solano-Agama¹, Alma Ortiz-Plata², María E. Mendoza-Garrido¹

¹Department of Physiology, Biophysics and Neurosciences,Center for Research and Advanced Studies (CINVESTAV-IPN), ²Department of Experimental Pathology,Nacional Institute of Neurology and Neurosurgery

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Summary

Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.