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Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment

Article DOI: 10.3791/54042-v • 08:24 min • June 12th, 2016
June 12th, 2016 •
1,2,3,4, 1,3, 1,3,4, 1,3,4
1Department of Anatomy & Structural Biology, Albert Einstein College of Medicine, 2Department of Radiology, Albert Einstein College of Medicine, 3Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine, 4Integrated Imaging Program, Albert Einstein College of Medicine

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This protocol describes the use of multiphoton microscopy to perform extended time-lapse imaging of multicellular interactions in real time, in vivo at single cell resolution.

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Time-lapse Intravital Imaging Multicellular Dynamics Tumor Microenvironment High Resolution Multiphoton Microscopy Vascular Permeability Kinetics Microscope Laser Components Heating Box Stage Insert Optical Contact Cover Glass Polyethylene Tubing 31 Gauge Needle Sterile PBS Syringe
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