Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

Dana Charuvi; Reinat Nevo; Ifat Kaplan-Ashiri; Eyal Shimoni; Ziv Reich

doi:10.3791/54066

Journal

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生物学

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通过低温扫描电子显微镜研究光合膜内冻结，割断叶片组织的超分子组织

$Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy$

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生物学

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JoVE 杂志生物学

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

通过低温扫描电子显微镜研究光合膜内冻结，割断叶片组织的超分子组织

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11,935 Views

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13:52 min

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June 23, 2016

DOI:

10.3791/54066-v

DOI:

10.3791/54066-v

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13:52 min

June 23, 2016

•

3 Views

Dana Charuvi^1,3, Reinat Nevo¹, Ifat Kaplan-Ashiri², Eyal Shimoni², Ziv Reich¹

¹Department of Biological Chemistry,Weizmann Institute of Science, ²Department of Chemical Research Support,Weizmann Institute of Science, ³Institute of Plant Sciences,Agricultural Research Organization, Volcani Center

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Summary

Here we describe a procedure for studying freeze-fractured plant tissues. High-pressure frozen leaf samples are freeze-fractured and double-layer coated, yielding well preserved frozen-hydrated samples that are imaged using the cryo-scanning electron microscope at high magnifications with minimal beam damage.