Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

James E. Baily; William C.W. Chen; Nusrat Khan; Iain R. Murray; Zaniah N. Gonz&#225;lez Galofre; Johnny Huard; Bruno P&#233;ault

doi:10.3791/54252

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Developmental Biology

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JoVE Journal Developmental Biology

Isolation of Perivascular Multipotent Precursor Cell Populations from Human Cardiac Tissue

从人类心脏组织血管周围多能前体细胞群的分离

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08:15 min

October 8, 2016

DOI: 10.3791/54252-v

James E. Baily¹, William C.W. Chen^2,3, Nusrat Khan⁴, Iain R. Murray⁴, Zaniah N. González Galofre⁴, Johnny Huard^5,6, Bruno Péault^4,7

¹Centre for Cardiovascular Science, Queen's Medical Research Institute,University of Edinburgh, ²Department of Bioengineering and Orthopaedic Surgery,University of Pittsburgh, ³Research Laboratory of Electronics and Department of Biological Engineering,Massachusetts Institute of Technology, ⁴MRC Centre for Regenerative Medicine,University of Edinburgh, ⁵Stem Cell Research Center, Department of Orthopaedic Surgery,University of Pittsburgh, ⁶Department of Orthopaedic Surgery,University of Texas Health Science Center at Houston, ⁷Department of Orthopaedic Surgery, UCLA Orthopaedic Hospital, David Geffen School of Medicine,University of California at Los Angeles

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人体心脏组织窝藏多能血管周围的前体细胞群可能适合于心肌再生。这里描述的技术允许与天然血管，即 CD146 ⁺ CD34相关联的两个多能基质干细胞群的同时分离和纯化^-周细胞和CD34 ⁺ CD146 ^-外膜细胞，从人的心肌。

该程序的总体目标是从人类心脏组织中分离两个离散的多能血管周围前体细胞群。这种方法可能有助于回答心脏再生领域的关键问题，例如血管周围细胞群的子集对这一过程的贡献。该技术的主要优点是它导致祖细胞群的异质性降低，并提高了所选主要的 ex 粘附技术的活力。

这项技术对缺血性心脏病的治疗具有意义，因为血管周围干细胞亚型已被证明具有心肌生成潜力。虽然这种方法可以深入了解血管周围细胞在心脏再生中的作用，但它也可以应用于心脏病其他方面的研究，例如心肌纤维化。一般来说，刚接触这种方法的个体会因为组织新鲜度而使用活细胞而感到挣扎。

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发育生物学第116 周细胞外膜细胞血管神经干细胞祖细胞心肌前体细胞心肌心脏再生流式细胞仪