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发育生物学

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5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平<em>体外</em

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation <em>In Vitro</em>

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发育生物学

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JoVE 杂志发育生物学

A 5-mC Dot Blot Assay Quantifying the DNA Methylation Level of Chondrocyte Dedifferentiation In Vitro

5-mC点印迹测定定量软骨细胞去分化的DNA甲基化水平<em>体外</em

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16,765 Views

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10:07 min

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May 17, 2017

DOI:

10.3791/55565-v

DOI:

10.3791/55565-v

•

10:07 min

May 17, 2017

•

10 Views

Zhaofeng Jia^1,2, Yujie Liang³, Bin Ma^4,5, Xiao Xu^2,6, Jianyi Xiong², Li Duan², Daping Wang^1,2

¹Guangzhou Medical University, ²Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), ³Department of Chemistry,The Chinese University of Hong Kong, ⁴School of Biomedical Engineering,Shanghai Jiao Tong University, ⁵Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, ⁶Shantou University Medical College

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Summary

我们提出了一种基于5-甲基胞嘧啶（5-mC）斑点印迹定量DNA甲基化的方法。我们在软骨细胞去分化中测定了5-mC的水平。这种简单的技术可用于快速测定ACI治疗中的软骨细胞表型。

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