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A Method For Production of Recombinant mCD1d Protein in Insect Cells.
JoVE 杂志
生物学
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JoVE 杂志 生物学
A Method For Production of Recombinant mCD1d Protein in Insect Cells.

A Method For Production of Recombinant mCD1d Protein in Insect Cells.

DOI:
10.3791/556-v

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09:41 min

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December 10, 2007

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,
1Department of Developmental Immunology,La Jolla Institute for Allergy and Immunology

Chapters

  • 00:00Title
  • 00:06Introduction
  • 02:08Thawing High 5 Insect Cells
  • 03:54Growing and Expanding Insect Cells
  • 05:50Titering Baculovirus
  • 07:11Infecting Insect Cells with Baculovirus
  • 08:46Conclusion

Summary

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A Method to prepare Insect cells and infect them with baculovirus for the the purpose of production of recombinant mCD1d proteinand generating mCD1d tetramers.

Tags

Recombinant MCD1d ProteinInsect CellsCD1 ProteinsAntigen-presenting MoleculesLipidsMHC Class IMHC Class IIBinding PocketsHydrophobic GrooveGlycolipidsNatural Killer T CellsAutoimmune DiseasesInfectious DiseasesTumor RejectionTetramersAlpha Galactosyl CeramideBiology Of NKT CellsLipid-reactive T Cells

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