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JoVE Journal Neuroscience
Modellering Neuronal død og forverring av musen primære lillehjernen Granule nerveceller
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Modellering Neuronal død og forverring av musen primære lillehjernen Granule nerveceller

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

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Denne protokollen beskriver en enkel metode for å isolere og dyrking hovedknappen på musen cerebral granule neurons (CGNs) fra 6-7 dag gammel pups, effektiv Albin på CGNs for tap og gevinst på funksjon studier, og modellering NMDA-indusert neuronal excitotoxicity, lav-kalium-indusert celledød, DNA-skader og oksidativt stress ved hjelp av samme kultur-modellen.

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Nevrovitenskap problemet 129 lillehjernen lillehjernen Granule Neurons (CGN) Adenovirus Lentiviral signaltransduksjon Excitotoxicity N-methyl-D-aspartate (NMDA) apoptose kalium klorid (KCl) Camptothecin Hydrogen peroxide (H2O2)
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