选择依赖和独立生成CRISPR / Cas9介导的哺乳动物细胞基因敲除
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11:35 min
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June 16, 2017
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Summary
Automatically generated
遗传操纵体细胞系的能力的最新进展对于基础和应用研究具有巨大的潜力。在这里，我们提出了CRISPR / Cas9在哺乳动物细胞系中产生敲除生产和筛选的两种方法，使用和不使用选择标记。
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Cite this Article
Sternburg, E. L., Dias, K. C., Karginov, F. V. Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells. J. Vis. Exp. (124), e55903, doi:10.3791/55903 (2017).
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