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JoVE Journal Neuroscience
Eksempel forberedelse til Endopeptidomic analyse i menneskelig Cerebrospinalvæske
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Eksempel forberedelse til Endopeptidomic analyse i menneskelig Cerebrospinalvæske

Article DOI: 10.3791/56244-v • 10:23 min • December 4th, 2017
December 4th, 2017 •
1, 1,2, 1, 1, 1, 1,2, 1,2,3, 1,2
1Inst. of Neuroscience and Physiology, Dept. of Psychiatry and Neurochemistry, Sahlgrenska Academy at University of Gothenburg, 2Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 3Department of Molecular Neuroscience, UCL Institute of Neurology

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En metode for masse spectrometric analyse av endogene peptider i menneskelig cerebrospinalvæske (CSF) vises. Ved å bruke molekylvekt cut-off filtrering, brukt kromatografiske før fraksjoneres, masse spectrometric analyse og en påfølgende kombinasjon av peptid identifikasjon strategier, var det mulig å utvide den kjente CSF peptidome nesten ti-fold sammenlignet tidligere studier.

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Nevrovitenskap problemet 130 Cerebrospinalvæske peptidomics endogen peptider biomarkers neurodegeneration Alzheimers sykdom LC--MS-/ MS pre fraksjoneres multiplekset isobar merking
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