/

/

用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

JoVE 杂志
生物化学

需要订阅 JoVE 才能查看此. 登录或开始免费试用。

JoVE 杂志生物化学

Rab10 Phosphorylation Detection by LRRK2 Activity Using SDS-PAGE with a Phosphate-binding Tag

用磷酸盐结合标记的 SDS-页 Rab10 磷酸化检测 LRRK2 活性

Please note that all translations are automatically generated. Click here for the English version.

14,884 Views

•

08:55 min

•

December 14, 2017

DOI:

10.3791/56688-v

DOI:

10.3791/56688-v

•

08:55 min

December 14, 2017

•

17 Views

Genta Ito¹, Taisuke Tomita^1,2

¹Laboratory of Brain and Neurological Disorders, Graduate School of Pharmaceutical Sciences,The University of Tokyo, ²Laboratory of Neuropathology and Neuroscience, Graduate School of Pharmaceutical Sciences,The University of Tokyo

成績單

Summary

本研究介绍了一种简单的方法检测内源性水平的 Rab10 磷酸化富亮氨酸重复激酶2。

Explore More Videos

Rab10 Phosphorylation LRRK2 Activity SDS-PAGE Phosphate-binding Tag Parkinson’s Research HEK293 Cells Cell Lysis Bradford Assay Sample Preparation SDS-PAGE Gel Manganese Chloride

Related Videos

Read Article