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JoVE Journal Neuroscience
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
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In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters

Article DOI: 10.3791/56765-v • 07:44 min • July 4th, 2018
July 4th, 2018 •
1,2, 2, 2
1PTN Joint Graduate Program, School of Life Sciences, Peking University, 2National Institute of Biological Sciences, Beijing

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This newly developed fluorescence-based technology enables long-term monitoring of the transcription of circadian clock genes in the suprachiasmatic nucleus (SCN) of freely moving mice in real-time and at a high temporal resolution.

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In Vivo Monitoring Circadian Clock Gene Expression Mouse Suprachiasmatic Nucleus Fluorescence Reporters Temporal Resolution Light-dark Conditions Dark-dark Conditions Optical Fiber Knife Ceramic Ferrule Methyl Ethyl Ketone Peroxide Cotton Swab Recombinant AAV Microsyringe Anesthetizing A Mouse Stereotaxic Apparatus Bregma Lambda Brain Periosteum
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