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杆状病毒基因治疗应用的生产与纯化

Production and Purification of Baculovirus for Gene Therapy Application

JoVE 杂志
遗传学

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JoVE 杂志遗传学

Production and Purification of Baculovirus for Gene Therapy Application

杆状病毒基因治疗应用的生产与纯化

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14,632 Views

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06:11 min

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April 09, 2018

DOI:

10.3791/57019-v

DOI:

10.3791/57019-v

•

06:11 min

April 09, 2018

•

13 Views

Md Nasimuzzaman^1,2, Johannes C.M. van der Loo^1,2,3, Punam Malik^1,2

¹Division of Experimental Hematology and Cancer Biology,Cincinnati Children’s Hospital Medical Center, ²University of Cincinnati College of Medicine, ³Raymond G. Perelman Center for Cellular and Molecular Therapeutics,The Children’s Hospital of Philadelphia

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Summary

在该协议中, 杆状病毒是通过瞬态转染杆状病毒质粒进入 Sf9 细胞并在无血清悬浮培养中扩增而产生的。用肝素亲和层析法纯化上清液, 进一步浓缩离心。该协议对用于基因治疗的杆状病毒的制备和纯化具有重要意义。

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