利用膜组分分离和GTP酶联免疫吸附测定法检测小GTP酶的异戊二烯化及GTP结合能力
9.6K 次观看
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被引用 6 次
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13:51 分钟
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2018年11月11日
DOI :10.3791/57646-v
2018年11月11日
•9.6K 次观看
Javad Alizadeh1,2 , Shahla Shojaei1,2,3 , Simone da Silva Rosa1 , Adel Rezaei Moghadam1,2 , Amir A. Zeki4 , Mohammad Hashemi5 , Marek J. Los6,7,8 , Joseph W. Gordon1,9 , Saeid Ghavami1,2,10
1Department of Human Anatomy and Cell Science, Rady Faculty of Health Sciences, Max Rady College of Medicine, University of Manitoba, 2Biology of Breathing Theme, Children Hospital Research Institute of Manitoba, University of Manitoba, 3Department of Biochemistry, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, 4Division of Pulmonary, Critical Care, and Sleep Medicine, Department of Internal Medicine, Center for Comparative Respiratory Biology and Medicine, 5Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, 6Department of Molecular Biology, School of Pharmacy with the Division of Laboratory Medicine in Sosnowiec, Medical University of Silesia, 7Centre de biophysique moléculaire - UPR 4301, Centre national de la recherche scientifique (CNRS) CS80054, 8LinkoCare Life Sciences AB, 9College of Nursing and Children's Hospital Research Institute of Manitoba, Rady Faculty of Health Sciences, University of Manitoba, 10Health Policy Research Center, Institute of Health, Shiraz University of Medical Sciences
本文介绍了一种研究Rho GTP酶法呢基化及鸟苷-5'-三磷酸（GTP）负载的实验方案。该方案包含两种详细方法，即膜组分分离和GTP酶联免疫吸附测定。本方案可用于检测其他多种小GTP酶的法呢基化状态及GTP结合情况。
Chapters in this video
0:00
标题
1:39
利用膜/胞质组分分离法测定RhoA定位
6:05
利用小G蛋白激活检测法测定RhoA GTP负载量
12:37
结果
13:09
结论
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