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成像 FITC-葡聚糖作为胞吐作用调节的记者

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

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生物学

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JoVE 杂志生物学

Imaging FITC-dextran as a Reporter for Regulated Exocytosis

成像 FITC-葡聚糖作为胞吐作用调节的记者

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12,529 Views

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04:50 min

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June 20, 2018

DOI:

10.3791/57936-v

DOI:

10.3791/57936-v

•

04:50 min

June 20, 2018

•

6 Views

Ofir Klein¹, Amit Roded¹, Koret Hirschberg², Mitsunori Fukuda³, Stephen J. Galli⁴, Ronit Sagi-Eisenberg¹

¹Department of Cell and Developmental Biology, Sackler Faculty of Medicine,Tel Aviv University, ²Department of Pathology, Sackler Faculty of Medicine,Tel Aviv University, ³Laboratory of Membrane Trafficking Mechanisms, Department of Developmental Biology and Neurosciences, Graduate School of Life Sciences,Tohoku University, ⁴Departments of Pathology and of Microbiology and Immunology and Sean N. Parker Center for Allergy and Asthma Research, School of Medicine,Stanford University

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Summary

在这里, 我们详细介绍了一种带电细胞成像的调节胞吐作用方法。该方法利用 FITC-葡聚糖, 在溶酶体相关细胞器中积累, 作为记者。这一简单的方法也允许区分不同模式的调节胞吐作用在细胞难以操纵基因。

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