使用阴离子交换树脂从生物气溶胶中检测病毒
7.9K 次观看
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06:10 分钟
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2018年8月22日
DOI :10.3791/58111-v
2018年8月22日
•7.9K 次观看
Joshua W. Schaeffer1 , Jeffrey C. Chandler2 , Margaret Davidson1,3 , Sheryl L. Magzamen1 , Alma Pérez-Méndez4 , Stephen J. Reynolds1 , Lawrence D. Goodridge5 , John Volckens6 , Alan B. Franklin2 , Susan A. Shriner2 , Bledar Bisha7
1High Plains Intermountain Center for Agricultural Health and Safety, Department of Environmental and Radiological Health Sciences, Colorado State University, 2National Wildlife Research Center, Wildlife Services, Animal and Plant Health Inspection Service, United States Department of Agriculture, 3Western Sydney University, 4Leprino Foods, Inc, 5Department of Food Science and Agricultural Chemistry, McGill University, 6Department of Mechanical Engineering, Colorado State University, 7Department of Animal Science, University of Wyoming
介绍了一种基于阴离子交换树脂的方法，该方法适用于基于液体冲击的生物气溶胶病毒采样。当与下游分子检测技术联用时，该方法可实现对生物气溶胶中病毒的简便且灵敏的检测。
Chapters in this video
0:04
Title
0:30
Bioaerosol Chamber Setup and Model Bioaerosolization Experiments
2:58
Nucleic Acid Extraction and qRT-PCR Detection
4:10
Results: Anion Exchange Resin Improves qRT-PCR-based, Negatively-charged Virus Bioaerosol Detection
4:46
Conclusion
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