Waiting
登录处理中...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Isolation of Physiologically Active Thylakoids…
JoVE Journal
Biochemistry

需要订阅 JoVE 才能查看此 内容.

JoVE Journal Biochemistry
Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Journal (Biochemistry)
An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
Journal (Biochemistry)
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
 

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays

Article DOI: 10.3791/58393-v • 12:25 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1, 1
1Department of Plant Biology, University of California - Davis

章节

总结概括
Automatic Translation

We present protocols herein for high-yield isolation of physiologically active thylakoids and protein transport assays for the chloroplast twin arginine translocation (cpTat), secretory (cpSec1), and signal recognition particle (cpSRP) pathways.

Tags

Thylakoids Protein Transport Assays Mechanism Energetics Location Of Proteins Control Of Environment Temperature PH Ionic Conditions Precursor Concentrations Peas Distilled Water Plastic Tray Vermiculite Growth Period Chloroplast Isolation Continuous Density Gradient Percoll 2xGB Buffer Centrifugation Pea Shoots 1xGB Buffer Blender Homogenization Filtration Cheese Cloth Centrifuge Tube
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter