Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

Yiyue Xu; Chun So; Hon-Ming Lam; Ming-Chiu Fung; Suk-Ying Tsang

doi:10.3791/58642

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Flow Cytometric Detection of Newly-formed Breast Cancer Stem Cell-like Cells After Apoptosis Reversal

细胞凋亡逆转后新形成乳腺癌干细胞样细胞的流式细胞仪检测

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11:21 min

January 26, 2019

DOI: 10.3791/58642-v

Yiyue Xu^1,2, Chun So¹, Hon-Ming Lam^1,2, Ming-Chiu Fung^1,2, Suk-Ying Tsang^1,2,3,4

¹School of Life Sciences,The Chinese University of Hong Kong, ²State Key Laboratory of Agrobiotechnology,The Chinese University of Hong Kong, ³Key Laboratory for Regenerative Medicine, Ministry of Education,The Chinese University of Hong Kong, ⁴Centre for Novel Biomaterials,The Chinese University of Hong Kong

Please note that some of the translations on this page are AI generated. Click here for the English version.

在这里, 我们提出了一个方案, 以分离凋亡乳腺癌细胞通过荧光激活细胞分类, 并进一步检测转变乳腺癌非干细胞到乳腺癌干细胞样细胞后, 细胞凋亡逆转流式细胞术。

凋亡逆转细胞真实种群的分离以前一直是个问题，这极大地限制了我们对凋亡逆转后果的理解。该协议允许我们生成更纯净的凋亡逆转细胞群。该技术的主要优点是，所用材料和设备在不同的机构中广泛提供。

这种技术很容易适用于隔离经过细胞凋亡逆转的更纯净的细胞群。如前所述，正常细胞也可以进行凋亡逆转，除了乳腺癌细胞，可以启动不同的化疗细胞，以及正常细胞，并可以使用各种凋亡刺激。由于逆转细胞经历了凋亡和快速分拣，恢复率通常较低。

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癌症研究第143期癌症干细胞乳腺癌凋亡凋亡逆转流式细胞术荧光激活细胞分选