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Isolation and Characterization of Human Umbilical Cord-derived Mesenchymal Stem Cells from Preterm and Term Infants

Article DOI: 10.3791/58806-v • 07:26 min • January 26th, 2019
January 26th, 2019 •
1, 2, 1, 1, 1, 1, 1, 1, 1, , 4, 1, 1, 1, 5, 1, 1
1Department of Pediatrics, Kobe University Graduate School of Medicine, 2Department of Pathology, Kobe Children's Hospital, 3Department of Pediatrics, Hyogo College of Medicine, 4Department of Developmental Pediatrics, Shizuoka Children's Hospital, 5Department of Pediatrics, Nihon University School of Medicine

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Human umbilical cord (UC) can be obtained during the perinatal period as a result of preterm, term, and postterm delivery. In this protocol, we describe the isolation and characterization of UC-derived mesenchymal stem cells (UC-MSCs) from fetuses/infants at 19-40 weeks of gestation.

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Isolation Characterization Human Umbilical Cord-derived Mesenchymal Stem Cells Preterm Infants Term Infants UCMSC Cell Therapy Isolation Technique Proliferation Intractable Diseases MSC Sources Adipose Tissue Synovium Skin Dental Pulp Umbilical Cord Dissection Mesenchymal Stem Cell Isolation Visual Demonstration Metal Tray Sterilized Scissors Forceps Pipettes (10ml And 25ml) Tissue Culture Dishes (60mm) PBS Purified Enzyme Blends Reduced Serum Medium Culture Medium
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