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Journal / Biochemistry / Identification of Orexin and Endocannabinoid…
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Biochemistry

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JoVE Journal Biochemistry
Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods
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Identification of Orexin and Endocannabinoid Receptors in Adult Zebrafish Using Immunoperoxidase and Immunofluorescence Methods

Article DOI: 10.3791/59308-v • 08:37 min • June 25th, 2019
June 25th, 2019 •
1,2, 3, 3, 2, 1
1Department of Sciences and Technologies (DST), University of Sannio, 2Endocannabinoid Research Group, Institute of Biomolecular Chemistry, 3Department of Veterinary Medicine and Animal Productions, University of Naples Federico II

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Presented here are protocols for immunohistochemical characterization and localization of orexin peptide, orexin receptors, and endocannabinoid receptors in the gut and brains of normal and diet-induced obesity (DIO) adult zebrafish models using immunoperixidase and double immunofluorescence methods.

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Orexin Receptors Endocannabinoid Receptors Zebrafish Immunoperoxidase Immunofluorescence Immunohistochemistry Molecular Markers Quantitative Analysis Larval Zebrafish Adult Zebrafish Standard Protocol Evolutionary Conservation Biomolecules Pathological Conditions Cryostat Tissue Blocks
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