利用斑马鱼幼虫作为宿主的胰腺癌患者来源异质性异种移植模型用于药物比较评估

1:28

Preparing the Equipment for the Microinjection

2:21

Preparing Embryos for Transplantation

2:56

Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue

4:56

Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately

6:29

Injecting Mixed Cell Suspension into the Zebrafish

7:10

Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities

8:11

Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae

9:12

Conclusion