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用于长期无细胞基因表达传导的多层微流体平台

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

JoVE 杂志
生物工程

This content is Free Access.

JoVE 杂志生物工程

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

用于长期无细胞基因表达传导的多层微流体平台

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10,192 Views

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11:23 min

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October 06, 2019

DOI:

10.3791/59655-v

DOI:

10.3791/59655-v

•

11:23 min

October 06, 2019

•

10148 Views

Ardjan J. van der Linden¹, Maaruthy Yelleswarapu², Pascal A. Pieters¹, Zoe Swank³, Wilhelm T. S. Huck², Sebastian J. Maerkl³, Tom F. A. de Greef^1,2

¹Institute for Complex Molecular Systems, Department of Biomedical Engineering, Computational Biology Group,Eindhoven University of Technology ²Institute for Molecules and Materials,Radboud University ³Institute of Bioengineering,School of Engineering École Polytechnique Fédérale de Lausanne (EPFL)

Summary

介绍了基于PDMS的多层微流体装置的制造过程，该装置允许长时间进行体外转录和翻译（IVTT）反应。此外，还全面概述了自动化和维护这些反应所需的硬件和软件，

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