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In Vitro Reconstitution of Spatial Cell Contact Patterns with Isolated Caenorhabditis elegans Embryo Blastomeres and Adhesive Polystyrene Beads

Article DOI: 10.3791/60422-v • 07:52 min • November 26th, 2019
November 26th, 2019 •
1,2, 1,2, 1,2
1Department of Zoology, The University of British Columbia, 2Life Sciences Institute, The University of British Columbia

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Tissue complexities of multicellular systems confound the identification of causal relationship between extracellular cues and individual cellular behaviors. Here, we present a method to study the direct link between contact-dependent cues and division axes using C. elegans embryo blastomeres and adhesive polystyrene beads.

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In Vitro Reconstitution Spatial Cell Contact Patterns Caenorhabditis Elegans Embryo Blastomeres Adhesive Polystyrene Beads Cellular Conduct Chemically Functional Polystyrene Beads Cellular Response C-Elegance Mouse Embryos Chlorite Treatment Blastomere Isolation Methods Carboxylate Modified Polystyrene Beads MES Buffer Bench-top Centrifuge EDAC Activation
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