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Cancer Research
利用活体中鸡胆膜研究妇科和泌尿癌
利用活体中鸡胆膜研究妇科和泌尿癌
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Using the Chicken Chorioallantoic Membrane In Vivo Model to Study Gynecological and Urological Cancers

利用活体中鸡胆膜研究妇科和泌尿癌

Full Text
11,057 Views
08:13 min
January 28, 2020

DOI: 10.3791/60651-v

Allison C. Sharrow1, Moe Ishihara1, Junhui Hu1, Il Hyun Kim1, Lily Wu1

1Molecular and Medical Pharmacology,University of California Los Angeles

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Please note that some of the translations on this page are AI generated. Click here for the English version.

我们提出了鸡胆膜模型作为替代的,可移植的,体内模型,用于妇科和泌尿细胞系和患者衍生肿瘤的移植。

将肿瘤包到鸡胆膜(CAM)上,可用于肿瘤原性、治疗效果或细胞相声的研究。此方法允许在体内免疫耐症环境中实现具有成本效益的快速肿瘤生长。使用CAM模型,可以使用细胞系甚至患者肿瘤标本快速测试新的治疗方法。

该协议最关键的方面是确保适当的 CAM 形成,并且在打开卵子时不会中断 CAM 或血管。在发育的第七天或第八天,当CAM已经完全发育时,关闭卵子旋转器,将大约一到三个鸡蛋放入生物安全柜的蛋架中。关灯后,将一个鸡蛋蜡烛器放在蛋壳上,以识别和标记一个鸡蛋的空气细胞的位置,然后将蛋烛台移动到蛋壳上,以找到一个大血管网络,并尽可能旋转鸡蛋。

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