通过2'，7'-二氯二氢氟辛二乙酸染色检测粘附细胞中的总活性氧物种
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04:53 min
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June 23, 2020
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Summary
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在这里，我们提出了一个协议，使用2'，7'-二氯二氢氟辛二乙酸酯（DCFH-DA）检测细胞活性氧物种（ROS）。2'，7'-二氯二氢氟酸酯（DCFH-DA）。该方法使用荧光显微镜可视化粘附细胞中的细胞ROS定位，并用荧光板读取器量化ROS强度。该协议简单、高效且经济高效。
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Cite this Article
Kim, H., Xue, X. Detection of Total Reactive Oxygen Species in Adherent Cells by 2',7'-Dichlorodihydrofluorescein Diacetate Staining. J. Vis. Exp. (160), e60682, doi:10.3791/60682 (2020).
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