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Inducing Hairy Roots by Agrobacterium rhizogenes-Mediated Transformation in Tartary Buckwheat (Fagopyrum tataricum)

Article DOI: 10.3791/60828-v • 08:12 min • March 11th, 2020
March 11th, 2020 •
1,2, 3, 3, 4, 1, 3, 5, 1, 1,2
1Key Laboratory of Beijing for Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, 2Artemisinin Research Center, China Academy of Chinese Medical Sciences, 3College of Life Science, Huaibei Normal University, 4Economic Crop Research Institute Sichuan Academy of Agriculture Sciences, 5Research Center of Buckwheat Industry Technology, Guizhou Normal University

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We describe a method of inducing hairy roots by Agrobacterium rhizogenes-mediated transformation in Tartary buckwheat (Fagopyrum tataricum). This can be used to investigate gene functions and production of secondary metabolites in Tartary buckwheat, be adopted for any genetic transformation, or used for other medicinal plants after improvement.

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Hairy Roots Agrobacterium Rhizogenes-mediated Transformation Tartary Buckwheat Genomics Microbiomics Dicot Plants Protocol Steps Visual Demonstration Guangtao Qian Sterilization Sodium Hypochlorite Seedlings Plant Tissue Culture Bottles MSSA Medium
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