一种用于小鼠角膜和结膜活体成像的定制多光子显微镜平台
5.4K 次观看
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被引用 1 次
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06:53 分钟
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2020年5月17日
DOI :10.3791/60944-v
2020年5月17日
•5.4K 次观看
1Department of Biomedical Engineering, National Taiwan University, 2Institute of Pharmacology, College of Medicine, National Taiwan University, 3Molecular Imaging Center, National Taiwan University, 4Department of Dermatology, National Taiwan University Hospital, and College of Medicine, 5Research Center for Developmental Biology and Regenerative Medicine, National Taiwan University, 6Department of Ophthalmology, Chang Gung Memorial Hospital, 7College of Medicine, Chang Gung University
* These authors contributed equally
本文展示了一种用于活体小鼠眼表成像的多光子显微成像平台。利用荧光转基因小鼠，可实现对眼表内的细胞核、细胞膜、神经纤维及毛细血管的可视化观察。来源于胶原结构的非线性二次谐波生成信号，可实现对基质结构的无标记成像。
Chapters in this video
0:04
Introduction
0:40
Multiphoton Microscopy Setup
1:29
Eye Holder Setup
2:38
Z-Serial Image Acquisition
3:43
Image Processing and 3D Reconstruction
4:36
Results: Representative Mouse Cornea and Conjunctiva Imaging
6:10
Conclusion
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