用于单个DNA分子大规模并行蛋白质合成的飞升级液滴阵列
10.3K 次观看
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被引用 2 次
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10:45 分钟
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2020年6月20日
DOI :10.3791/60945-v
2020年6月20日
•10.3K 次观看
1SUGAR Program, X-star, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 2Center for Mathematical Science and Advanced Technology, Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Deep-Sea Nanoscience Research Group, Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
本实验方案的总体目标是在1 cm2的平面基底上制备超过一百万个有序、均一、稳定且具有生物相容性的飞升级液滴，可用于无细胞蛋白合成。
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Chapters in this video
0:04
Introduction
0:33
Cleaning and Silanizing the Glass
1:26
Spin-Coating the Glass Substrate
2:40
Photolithography, Etching, and Cleaning
3:47
Fabricating the Microchamber Array Device
5:04
Generating the Femtoliter Droplet Array (FemDA)
6:35
Image Data Analysis
8:45
Results: Cell-Free Protein Synthesis in FemDA
10:07
Conclusion
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