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JoVE Journal
Immunology and Infection

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Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice
 

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice

Article DOI: 10.3791/61454-v 08:17 min August 7th, 2020
August 7th, 2020

章节

总结概括

This protocol describes a chronic cranial window implantation technique that can be used for longitudinal imaging of neuro-glio-vascular structures, interactions, and function in both healthy and diseased conditions. It serves as a complementary alternative to the transcranial imaging approach that, while often preferred, possesses some critical limitations.

Tags

Precise Brain Mapping In Vivo Imaging Neuro-immune Dynamics Mice Two-photon Microscopy Cellular Dynamics Brain Plasticity Neurodegeneration Cranial Window Implantation CX3CR1 GFP Heterozygote Mouse Stereotactic Surgery Station Bupivacaine Dexamethasone Betadine Alcohol
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