糖精中遗传链接调控时间寿命的抑制 器屏幕
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10:39 min
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September 17, 2020
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这里是一个协议，通过增加的拷贝数抑制器屏幕在 糖精中识别遗传相互作用。这种方法允许研究人员识别、克隆和测试短寿命酵母突变体中的抑制器。我们测试S SIR2拷贝数 增加对自 噬空突变体寿命的影响。
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Cite this Article
Dix, C., Sgro, S., Patel, A., Perrotta, C., Eldabagh, N., Lomauro, K. L., Miguez, F. W., Chohan, P., Jariwala, C., Arnone, J. T. A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae. J. Vis. Exp. (163), e61506, doi:10.3791/61506 (2020).
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