Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

Raven Shah; Shuiyun Lan; Maritza  N. Puray-Chavez; Dandan Liu; Philip  R. Tedbury; Stefan G. Sarafianos

doi:10.3791/61843

Journal

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生物学

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单细胞多重荧光成像，可可视化病毒核酸和蛋白质，并监测 HIV、HTLV、HBV、HCV、寨卡病毒和流感感染

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

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生物学

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JoVE 杂志生物学

Single-Cell Multiplexed Fluorescence Imaging to Visualize Viral Nucleic Acids and Proteins and Monitor HIV, HTLV, HBV, HCV, Zika Virus, and Influenza Infection

单细胞多重荧光成像，可可视化病毒核酸和蛋白质，并监测 HIV、HTLV、HBV、HCV、寨卡病毒和流感感染

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2,727 Views

•

07:24 min

•

October 29, 2020

DOI:

10.3791/61843-v

DOI:

10.3791/61843-v

•

07:24 min

October 29, 2020

•

2705 Views

Raven Shah*¹, Shuiyun Lan*¹, Maritza N. Puray-Chavez², Dandan Liu², Philip R. Tedbury¹, Stefan G. Sarafianos¹

¹Laboratory of Biochemical Pharmacology, Department of Pediatrics,Emory University School of Medicine ²Department of Molecular Microbiology & Immunology,University of Missouri School of Medicine

Summary

这里介绍的是荧光成像方法的 协议，multiplex immunofluorescent cELL 基于d etection of DNA， RNA和 Protein（MICDDRP），该方法能够同时对不同类型和链的病毒蛋白和核酸进行荧光单细胞可视化。这种方法可以应用于各种系统。

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Single-Cell Multiplexed Fluorescence Imaging Viral Nucleic Acids Viral Proteins HIV Detection HTLV Detection HBV Detection HCV Detection Zika Virus Detection Influenza Infection Monitoring Multiplex Immunofluorescence Cell-based Detection DNA Visualization RNA Visualization Protein Visualization Poly-D-lysine Coating Hybridization Buffer

单细胞多重荧光成像，可可视化病毒核酸和蛋白质，并监测 HIV、HTLV、HBV、HCV、寨卡病毒和流感感染

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