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Sand Fly (Phlebotomus papatasi) Embryo Microinjection for CRISPR/Cas9 Mutagenesis

Article DOI: 10.3791/61924-v • 05:44 min • November 17th, 2020
November 17th, 2020 •
1, 1, 1, 1, 2, 2
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research

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This protocol details the steps of CRISPR/Cas9 targeted mutagenesis in sand flies: embryo collection, injection, insect rearing, and identification as well as selection of mutations of interest.

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Sand Fly Phlebotomus Papatasi CRISPR/Cas9 Mutagenesis Targeted Mutagenesis Embryo Microinjection Insect Genome Editing Sand Fly Embryos Humidity Sensitivity Blood Feeding Sucrose Solution Egg-laying Chamber Filter Paper Mouth Aspirator Petri Dish Stereo Microscope Paint Brush
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