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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Article DOI: 10.3791/62009-v • 10:51 min • April 23rd, 2021
April 23rd, 2021 •
1, 2, 3, 4, 5, 4, 1
1Department of Surgery, Louisiana State University Health Sciences Center, 2Department of Bioinnovation, Tulane University, 3Tulane University School of Medicine, 4Department of Biological and Agricultural Engineering, Louisiana State University, 5Department of Medicine, Tulane University School of Medicine

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This protocol describes the construction of an in vitro microphysiological system for studying breast cancer using primary human breast tissue with off the shelf materials.

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Modeling Breast Cancer Human Breast Tissue Microphysiological System Ex Vivo Interactions Breast Adipocytes Immune Cells Vascular Structures Ductal Structures Extracellular Matrix Personalized Medicine Pharmaceutical Development Tumor Initiation Fibrosis Extracellular Matrix Remodeling Gelatin Solution Serological Pipette Six Well Plate PNIPAAm Coded ASC Plates
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