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Dynamic Imaging of Chimeric Antigen Receptor T Cells with [18F]Tetrafluoroborate Positron Emission Tomography/Computed Tomography

Article DOI: 10.3791/62334-v • 09:34 min • February 17th, 2022
February 17th, 2022 •
1,2, 1,2, 3, 1,2,4,5, 1,2, 3, 3, 3, 3, 1,2, 1,2,5,6
1T Cell Engineering, Mayo Clinic, 2Division of Hematology, Mayo Clinic, 3Department of Radiology, Mayo Clinic, 4Regenerative Sciences PhD, Mayo Clinic, 5Department of Molecular Medicine, Mayo Clinic, 6Department of Immunology, Mayo Clinic

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This protocol describes the methodology for non-invasively tracking T cells genetically engineered to express chimeric antigen receptors in vivo with a clinically available platform.

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Chimeric Antigen Receptor T Cells [18F]tetrafluoroborate Positron Emission Tomography/computed Tomography Sodium-iodide Symporter PET Scan TLP PET Imaging CAR T-cell Therapy T-cell Trafficking Expansion Peripheral Blood Mononuclear Cells (PBMCs) Density Gradient Technique
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