Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Joann Phan; Joseph Kapcia III; Cynthia I. Rodriguez; Victoria L. Vogel; Daniel B Cardin; Sage J. B. Dunham; Katrine Whiteson

doi:10.3791/62547

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

通过真空辅助吸附剂提取从人类相关样品中捕获活性产生的微生物挥发性有机化合物

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09:19 min

June 1, 2022

DOI: 10.3791/62547-v

Joann Phan¹, Joseph Kapcia III¹, Cynthia I. Rodriguez², Victoria L. Vogel³, Daniel B Cardin³, Sage J. B. Dunham³, Katrine Whiteson¹

¹Department of Molecular Biology and Biochemistry,University of California Irvine, ²Department of Ecology and Evolutionary Biology,University of California Irvine, ³Entech Instruments Inc.

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该协议描述了使用真空辅助吸附剂提取方法从生物样品中提取挥发性有机化合物，使用Entech样品制备导轨进行气相色谱结合质谱分析以及数据分析。它还描述了生物样品的培养和稳定同位素探测。

该协议使我们能够轻松地浓缩和鉴定各种生物样品中微生物的挥发性代谢物和活性产生的挥发性挥发物。VASE是一种更人性化的浓缩低丰度挥发物的方法。您所需要的只是在近真空下提供一个样本，剩下的就让物理学来做。

获得临床样品的挥发性分析对于在许多不同的疾病状态下发现代谢生物标志物具有重要意义。例如，病原体驱动和感染或抗菌治疗的成功可以通过唾液，痰液或呼吸样本的挥发性分析来检测。为了制备粪便样品，在1.5毫升微量离心管中向100毫克粪便中加入一毫升去离子水并涡旋三分钟。

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生物化学第184期挥发性有机化合物 GC-MS 真空辅助吸附剂提取顶空提取临床样品稳定同位素探测