使用同质珠为基础的检测的查佩龙-科查佩龙相互作用研究
2,763 Views
•
06:51 min
•
July 21, 2021
DOI:
Summary
Automatically generated
该协议提出了一种利用谷胱甘肽相关的供体珠与 GST 融合的 TPR-motif 共同伴奏和接受珠以及 Hsp90 衍生肽来探索蛋白质- 蛋白质相互作用的技术。我们使用此技术筛选小分子，以破坏 Hsp90-FKBP51 或 Hsp90-FKBP52 相互作用，并识别出有效和选择性的 Hsp90-FKBP51 相互作用抑制剂。
Related Videos
08:32
Detection of the pH-dependent Activity of Escherichia coli Chaperone HdeB In Vitro and In Vivo
Related Videos
10609 Views
10:24
Defining Hsp33's Redox-regulated Chaperone Activity and Mapping Conformational Changes on Hsp33 Using Hydrogen-deuterium Exchange Mass Spectrometry
Related Videos
8675 Views
00:07
Quantification of Protein Interaction Network Dynamics using Multiplexed Co-Immunoprecipitation
Related Videos
8341 Views
12:22
Biomimetic Materials to Characterize Bacteria-host Interactions
Related Videos
9341 Views
08:16
In Vitro Characterization of Histone Chaperones using Analytical, Pull-Down and Chaperoning Assays
Related Videos
2743 Views
07:03
Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions
Related Videos
2873 Views
09:40
Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes
Related Videos
15112 Views
00:08
In Situ Monitoring of Transiently Formed Molecular Chaperone Assemblies in Bacteria, Yeast, and Human Cells
Related Videos
7058 Views
06:55
Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
Related Videos
2883 Views
02:07
Amplified Luminescent Proximity Homogeneous Assay: A Bead-Based Proximity Assay to Screen Small Molecules Inhibiting Protein-Protein Interactions
Related Videos
194 Views
Read Article
Cite this Article
Wang, L., Bergkvist, L., Kumar, R., Winblad, B., Pavlov, P. F. Studies of Chaperone-Cochaperone Interactions using Homogenous Bead-Based Assay. J. Vis. Exp. (173), e62762, doi:10.3791/62762 (2021).
Copy