液相色谱联用折射率或质谱检测用于基于裂解液的无细胞体系中的代谢物分析

1:08

Starting, Stopping, and Processing Time Course CFME Reactions for HPLC-RID Quantification

3:18

Creating a Sequence Table for Autosampling and Start the HPLC-RID System for Data Acquisition

4:54

Extracting and Analyzing Data Post-Run

7:20

Starting, Stopping, and Processing Time Course Isotope Tracing CFME Reactions for LC-MS/MS Quantification

9:20

Setting Up a Run Sequence and Starting the LC-MS/MS Run

10:16

Calculating Negative Mode Masses of 13C-Labeled Glucose-Derived Metabolites and Searching for the m/z Features of These Analytes in Filtered Data

11:45

Results: Metabolite Profiling in Lysate-based Cell-free Systems

13:54

Conclusion