双色荧光互相关光谱研究活细胞中蛋白质-蛋白质相互作用和蛋白质动力学
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December 11, 2021
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Summary
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我们提出了一种实验方案和数据分析工作流程，以执行活细胞双色荧光交叉相关光谱（FCCS）与Förster共振能量转移（FRET）相结合，以使用现代荧光标记技术研究活细胞中的膜受体动力学。
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Cite this Article
Hemmen, K., Choudhury, S., Friedrich, M., Balkenhol, J., Knote, F., Lohse, M. J., Heinze, K. G. Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells. J. Vis. Exp. (178), e62954, doi:10.3791/62954 (2021).
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