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人鼻上皮类器官的培养和成像
JoVE 杂志
医学
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JoVE 杂志 医学
Culture and Imaging of Human Nasal Epithelial Organoids
DOI:

13:20 min

December 17, 2021

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Chapters

  • 00:04Introduction
  • 01:40Cell Culture and Expansion of Nasal Epithelial Cells
  • 04:23Growth and Differentiation of Organoids in Slides and Culture Inserts
  • 06:06Isolation and Fixation of Organoids
  • 07:36Preparation and Isolation of Organoids for Histological Sectioning
  • 08:57Baseline Lumen Measurements
  • 09:56Results: Live and Fixed Imaging of the Airway Epithelial Organoids Derived from Nasal Epithelial Cells and Analysis Methods
  • 12:21Conclusion

Summary

自动翻译

这里提出了一个详细的方案来描述来自人类鼻上皮细胞的 体外 类器官模型。该协议具有需要标准实验室设备的测量选项,以及用于专用设备和软件的其他可能性。

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