原代人类鼻上皮细胞模型的采集、扩增与分化及其用于纤毛搏动频率的定量分析
4.8K 次观看
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被引用 9 次
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11:13 分钟
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2021年11月10日
DOI :10.3791/63090-v
2021年11月10日
•4.8K 次观看
Katelin M. Allan1,2 , Sharon L. Wong1,2 , Laura K. Fawcett1,2,3 , Alexander Capraro1,2 , Adam Jaffe1,2,3 , Cristan Herbert4 , Elvis Pandzic5 , Shafagh A. Waters1,2,3
1School of Women's and Children's Health, Faculty of Medicine and Health, University of New South Wales, 2Molecular and Integrative Cystic Fibrosis Research Centre (miCF RC), Faculty of Medicine and Health, University of New South Wales, 3Department of Respiratory Medicine, Sydney Children's Hospital, 4Department of Pathology, School of Medical Sciences, University of New South Wales Australia, 5Katharina Gaus Light Microscopy Facility, Mark Wainwright Analytical Centre, University of New South Wales
本方案描述了鼻上皮细胞的采集、扩增和分化为类器官型气道上皮细胞模型的过程，并通过活细胞成像和自定义脚本对纤毛摆动频率进行定量分析。
Chapters in this video
0:05
Introduction
1:16
Collection of Nasal Inferior Turbinate Brushings
2:25
Airway Epithelial Cell Expansion and Maintenance
3:23
Seeding and Differentiation of Airway Epithelial Cells and Maintenance of Differentiated ALI Models
4:12
Three-Dimensional Airway Epithelial Organoids
5:54
Imaging Cilia Beat Frequency
7:18
Data Analysis and Quantification of Cilia Beat Frequency
9:20
Results: Cilia Beat Frequency in Airway Epithelial Cell ALI Models of Cystic Fibrosis Patients and Healthy Participants
10:36
Conclusion
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