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Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments

Article DOI: 10.3791/63128-v • 09:12 min • October 4th, 2021
October 4th, 2021 •
*1, *1, 1, 1, 1, 1,2
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, 2Division of Cardiology, Georgia Institute of Technology and Emory University
* These authors contributed equally

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We present a method for isolating endothelial cells and nuclei from the lumen of mouse carotid arteries exposed to stable or disturbed flow conditions to perform single-cell omics experiments.

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Isolation Endothelial Cells Lumen Mouse Carotid Arteries Single-Cell Multi-Omics Experiments Molecular Pathways Therapeutics Acute Disturbed Flow Chronic Disturbed Flow Atherosclerosis Target Genes Potential Therapeutics Carotid Artery Isolation Buprenorphine Betadine Isopropanol Solution Ventral Midline Incision LCA Branch Point Ligate Arteries Superior Thyroid Artery Tissue Glue Sutures Recovery Cage Hypothermia IV Line Left Ventricle
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