原代人鼻上皮细胞:精准医学背景下的生物样本库
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08:35 min
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April 22, 2022
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在这里，我们描述了原代人鼻上皮（HNE）细胞在气液界面处的分离，扩增和分化，以及允许成功冷冻和解冻扩增的HNE的生物样本库方案。该方案分析了不同调节剂处理下分化HNE细胞的电生理特性和CFTR相关氯离子分泌校正。
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Cite this Article
Kelly, M., Dreano, E., Hatton, A., Lepissier, A., Golec, A., Sermet-Gaudelus, I., Pranke, I. Primary Human Nasal Epithelial Cells: Biobanking in the Context of Precision Medicine. J. Vis. Exp. (182), e63409, doi:10.3791/63409 (2022).
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