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一种用于评估细胞DNA损伤的高通量彗星检测方法

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

JoVE 杂志
生物学

This content is Free Access.

JoVE 杂志生物学

A High-Throughput Comet Assay Approach for Assessing Cellular DNA Damage

一种用于评估细胞DNA损伤的高通量彗星检测方法

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4,857 Views

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07:57 min

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May 10, 2022

DOI:

10.3791/63559-v

DOI:

10.3791/63559-v

•

07:57 min

May 10, 2022

•

4830 Views

Yunhee Ji*¹, Mahsa Karbaschi*², Abdulhadi Abdulwahed*³, Natalia S. Quinete⁴, Mark D. Evans⁵, Marcus S. Cooke¹

¹Oxidative Stress Group, Dept. Cell Biology, Microbiology and Molecular Biology, College of Arts and Sciences,University of South Florida ²Cepheid (Danaher Corp.),US Technical Operations ³Department of Clinical Laboratory Sciences, College of Applied Medical Sciences,King Saud University ⁴Department of Chemistry and Biochemistry, Institute of Environment,Florida International University ⁵Leicester School of Allied Health Sciences, Faculty of Health and Life Sciences,De Montfort University

Summary

彗星测定是检测DNA损伤的常用方法。本研究描述了一种在彗星测定的代表性变体中运行载玻片的方法。这种方法显著增加了样品数量，同时减少了分析运行时间、玻片操作次数和凝胶损坏风险。

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