全基因组范围转录因子-DNA 结合相互作用分析：一种针对Candida albicans的全面CUT&RUN方法与数据分析流程
3.6K 次观看
•
被引用 9 次
•
07:48 分钟
•
2022年4月1日
DOI :10.3791/63655-v
2022年4月1日
•3.6K 次观看
Mohammad N. Qasim*1,2 , Ashley Valle Arevalo*1,2 , Akshay D. Paropkari*1,2 , Craig L. Ennis1,2,4 , Suzanne S. Sindi3 , Clarissa J. Nobile1,4 , Aaron D. Hernday1,4
1Department of Molecular and Cell Biology, University of California, Merced, 2Quantitative and Systems Biology Graduate Program, University of California, Merced, 3Department of Applied Mathematics, University of California, Merced, 4Health Sciences Research Institute, University of California, Merced
* These authors contributed equally
本方案描述了一种在人类真菌病原体Candida albicans中应用靶点切割释放核酸酶技术（CUT&RUN）的实验方法及数据分析流程。
探索更多视频
Chapters in this video
0:04
Introduction
0:49
Isolation of Nuclei and Primary Antibody Binding
2:40
Binding of pAG-MNase to Antibody
3:36
Library Preparation for Sequencing
5:22
CUT&RUN Sequence Analysis
5:54
Results: Quality Control Steps and Evaluation of Ndt80 and Efg1 Enriched Peaks Using CUT&RUN Protocol
7:22
Conclusion
Related Videos