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发育生物学
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Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage
JoVE 杂志
发育生物学
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JoVE 杂志 发育生物学
Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

Generation of Mesenchymal Stem Cells from Human Umbilical Cord Tissue and their Differentiation into the Skeletal Muscle Lineage

DOI:
10.3791/63725-v

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07:27 min

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August 31, 2022

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,
1Translational Health Science and Technology Institute (THSTI),NCR Biotech Science Cluster

Chapters

  • 00:04Introduction
  • 01:06Isolation of MSCs from Cord Tissue and Immunophenotyping of uMSCs
  • 03:51Differentiation of Mesenchymal Stem Cells from uMSCs into Skeletal Muscle
  • 05:00Results: Analysis and Characterization of uMSCs
  • 06:41Conclusion

Summary

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We describe a protocol for the isolation of mesenchymal stem cells from human umbilical cord tissue and their differentiation into the skeletal muscle lineage.

Tags

Mesenchymal Stem CellsSkeletal Muscle LineageCord TorsionWharton’s JellyEndothelial CellsGrowth MediumMyogenic Differentiation

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