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结核分枝杆菌 通过尺寸排阻色谱实现细胞外囊泡富集
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免疫与感染
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JoVE 杂志 免疫与感染
Mycobacterium tuberculosis Extracellular Vesicle Enrichment through Size Exclusion Chromatography
DOI:

00:00 min

May 19, 2022

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Chapters

  • 00:04Introduction
  • 00:50Preparation of Crude Mtb EV Concentrate
  • 02:36Size Exclusion Chromatography for the Enrichment of Mtb EVs from CFP
  • 05:52Quantification and Qualification of the Mtb EVs
  • 07:25Results: Silver Stain and Western Blots by Fraction, TEM Images, and NTA Distribution
  • 08:42Conclusion

Summary

自动翻译

该方案描述了尺寸排阻色谱,这是一种从培养物上清液中富集 结核分枝杆菌 细胞外囊泡的简单且可重复的技术。

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