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Biochemistry

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Single-Particle Cryo-EM-datainsamling med steglutning med Leginon
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Single-Particle Cryo-EM-datainsamling med steglutning med Leginon

Article DOI: 10.3791/64136-v • 04:52 min • July 1st, 2022
July 1st, 2022 •
1, 1, 2, 2,3, 1,4,5
1Laboratory of Genetics, The Salk Institute for Biological Studies, 2Jack H. Skirball Center for Chemical Biology and Proteomics, The Salk Institute for Biological Studies, 3Department of Chemistry and Biochemistry, University of California San Diego, 4Graduate School of Biological Sciences, Section of Molecular Biology, University of California San Diego, 5Department of Integrative Structural and Computational Biology, The Scripps Research Institute

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Detta protokoll beskriver ett generaliserat och lättimplementerat schema för insamling av lutade enpartikeldata i kryo-EM-experiment. Ett sådant förfarande är särskilt användbart för att erhålla en högkvalitativ EM-karta för prover som lider av preferensorienteringsbias på grund av vidhäftning till luft-vattengränssnittet.

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Denna månad i JoVE nummer 185
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